Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate
نویسندگان
چکیده
منابع مشابه
Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملheterologous expression of potato virus y coat protein, isolate pot187
background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
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Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated. Objectives: An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course...
متن کاملPurification and characterization of antiviral protein from silkworm fecal matter
Antiviral proteins (AVP), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (NPV) in vitro and in vivo. The extract of silkworm fecal matter prepared in phosphate buffer solution of pH 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich AVP, then which was dialyzed. The dialysate was applied to the column containing silica gel-G, the column e...
متن کاملserological methods to confirm expression of coat protein gene from an iranian isolate of cucumber mosaic virus in escherichia coli
conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2010
ISSN: 1743-422X
DOI: 10.1186/1743-422x-7-34